By Dr. Jeff Golini, Founder & Executive Scientist
I am sure you are now aware of the dozen or more class-action lawsuits filed against various national brand sports nutrition companies for protein spiking. And I’m told there are many more to come. This is a direct result of not using proper protein spiking detection methods to prevent it.
This is not a new problem. I first discovered this unethical practice back in the early 1990s when several companies got caught by the FDA cutting protein with cheap fillers like sugar and maltodextrin. The government’s response back then? A slap on the wrist. Those companies barely paused before continuing their deceptive practices.
Today, the game has changed, but the greed hasn’t. The new fad, cynically called “protein spiking” or “nitrogen spiking,” is more sophisticated—and in some ways, more deceptive. It involves adding cheap free-form amino acids, such as glycine, taurine, or glutamine, to a protein powder. Why? To artificially boost the nitrogen levels detected by standard protein tests, like the Kjeldahl or Dumas methods. This is precisely why reliable protein spiking detection methods, such as the FTNIR technology we use at All American Pharmaceutical, are essential for protecting brand integrity and consumer trust.
Here’s how the scam works: These conventional tests don’t actually measure protein directly. They measure total nitrogen and then use a calculation (multiplying by a factor like 6.25) to estimate protein content. When you spike a powder with nitrogen-rich amino acids, the test registers a higher “protein” value than what’s actually present as complete, functional protein. The manufacturer saves money by substituting expensive whey or casein with cheap amino acids, and the consumer ends up with a product that delivers far less real protein than the label claims.
The protein powder industry is a multi-billion-dollar business here in the United States. The temptation to cut corners is enormous. I see it every day. It is incredibly disheartening to have a potential customer ask me for a bid on whey protein, only to tell me he’s currently paying half of my actual costs. My response is always the same: “Send me a sample of what you’re buying. Let me test it.”
And guess what? Nine times out of ten, the sample comes back spiked. It’s been adulterated with free-form amino acids or other nitrogenous compounds. The label might say “100% Whey Protein Isolate,” but the contents of the bag are something entirely different. This practice is not just a minor infraction; it’s illegal, it’s unethical, and it flagrantly disregards every Current Good Manufacturing Practice (cGMP) regulation mandated by the FDA.
The Science of Honesty: How We Detect Protein Spiking
So, how do we separate the real from the fake? At All American Pharmaceutical, we don’t rely on the outdated nitrogen-testing methods that spikers exploit. We use Fourier Transform Near-Infrared Spectroscopy (FTNIR), specifically employing BOM (Basic Oil Molecule) Cluster Analysis.
Think of FTNIR as creating a unique molecular fingerprint for a substance. A sample of 100% pure, unadulterated whey protein has a specific, complex spectral fingerprint that reflects its true peptide bonds and protein structure. A sample of whey that has been spiked with free-form amino acids will have a different fingerprint. The amino acids, being smaller and simpler molecules, distort that fingerprint in predictable ways.
Our BOM Cluster Analysis software allows us to compare a sample against a vast library of known, pure standards. It doesn’t just detect if something is present; it can identify the pattern of adulteration. It can see through the smoke and mirrors. I have attached my cluster analysis and spectra reports below. These aren’t just lines on a graph; they are the evidence. They show, with scientific certainty, how easy it is to visually and mathematically separate adulterated protein from 100% real protein. This isn’t subjective opinion; it’s objective, reproducible science.
The Real Cost of Protein Spiking
I cannot understand how the people running these companies sleep at night, knowing they are systematically cheating consumers who trust them. They are stealing money from hard-working people who are trying to fuel their bodies, support their training, and improve their health.
This class action investigation is a step in the right direction. It seeks to put an end to this deceptive practice and, hopefully, level the playing field for manufacturers like us who refuse to compromise. But lawsuits alone won’t solve the problem. The real power lies with you—the consumer, the brand owner, the retailer.
If consumers stopped purchasing products from these companies and retailers stopped supporting their brands, this cheating would stop almost overnight. The market would correct itself. Demand honesty, demand transparency, and demand proof.
At All American Pharmaceutical, we offer that proof. Our quality assurance protocols, anchored by advanced FTNIR technology and our team’s decades of experience, ensure that every protein powder bearing our brand—or a partner’s—is exactly what it claims to be: pure, potent, and unspiked.
When you choose to manufacture with us, you’re not just getting a production partner. You’re aligning with a company that has spent over 40 years fighting for integrity in this industry. We provide the tools, the testing, and the expertise to protect your brand’s reputation. We make sure that your “protein” is protein, so you can build a brand that lasts.
The use of NIR to determine if a protein is pure or spiked.
All American Pharmaceutical utilizes FTNIR (Fourier Transform Near Infrared Spectroscopy) using the BOM Cluster Analysis. Below, you can download Dr. Golini’s cluster analysis and spectra reports showing how easy it is to separate adulterated protein from 100% real protein.
DOWNLOAD: 2014 Spiked Protein Part 2 Spectra
(You can read Part 1 of this article here.)

