At All American Pharmaceutical, our research extends beyond sports nutrition into fundamental cellular mechanisms. We conducted this Kre-Celazine tumor cell study to investigate whether the alkali-buffered fatty acids in our proprietary compound could influence abnormal cell proliferation. The results, showing greater anti-proliferative effects for buffered versus non-buffered fats, open new avenues for understanding how pH modification may impact cellular health.
Buffered Vs Non-Buffered Aliphatic Fatty Acids and Their Anti-Proliferative Effects in Human Tumor Cell Lines
Jeff Golini, Wendy Jones, MS
All American Pharmaceutical, Billings, MT, and Royal Knight, Incorporated, Rochester, MN
Background
Tumor cells generate a micro-acidic environment due to increased fermentative metabolism and poor perfusion. This phenomenon, known as the Warburg effect, reflects a fundamental metabolic shift in cancer cells. It is believed that this action creates a lower pH environment, which promotes invasive tumor growth in primary and metastatic cancers by enhancing tissue remodeling and immune evasion.
Dietary fats, saturated and unsaturated, have been shown to profoundly influence cell membrane fluidity, receptor function, and signaling pathways. Certain long-chain, saturated and unsaturated fatty acids, some belonging to the omega-5 and omega-9 fatty acid classes, have demonstrated the ability to inhibit abnormal cell proliferation in some tumor models through mechanisms involving apoptosis and cell cycle arrest.
A low pH has been shown to increase the release of an important matrix-remodeling protease in a mouse tumor model, thereby facilitating metastasis. When the pH environment was increased using sodium bicarbonate (NaHCO(3)), a significant reduction in tumor growth and invasiveness was observed, suggesting that pH modulation alone can alter cancer progression. This provides a mechanistic rationale for investigating buffered compounds like Kre-Celazine®.
Study Objective
The antiproliferative effects of saturated and unsaturated fatty acids, both buffered and non-buffered, were investigated in a panel of tumor cell lines.
Participants and Method
Juveniles less than 17 years of age, with confirmed JRA/JIA, unresponsive to standard anti-inflammatory therapy, and who demonstrated palpable signs of inflammation, restricted range of motion, elevated inflammatory markers: CRP, ANA, and accelerated ESR.
Each participant received two 750 mg capsules (total, 1,500 mg) of Kre- Celazine®. This dose was taken daily (one in the morning and one in the evening) on an empty stomach, only with water, for 30 consecutive days.
Each family was provided with a Pain Journal to be completed weekly, indicating the perceived level of physical discomfort for each afflicted joint. At the conclusion of the 30-day treatment period, each individual returned to the hospital for re-examination and a fasting blood draw.
Results
(Detailed numbers can be seen by clicking the Research Poster Presentation image below)
Summary
The alkali-buffered fats exerted a greater antiproliferative effect than the non- buffered fats throughout the panel of malignant cells tested.
Research Poster Presentation Image:
